The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. ATP standard curves read on SpectraMax microplate readers. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Download PDF. Learn more about its features, specifications, and applications in this user. 2. The screw remains inside the instrument. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. 300 µL of Detergent Compatible Bradford reagent was added to each well. their consistent performance, reliability and durability. No accessories are needed and SoftMax® Pro Software. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Figure 1. Compact designs. Temperature control. Italic and boldface type are used for emphasis. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Sensitive fluorescent quantitation of DNA with the Quant-iT. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. Add to Cart. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. For SpectraMax ABS Plus reader, r 2 = 0. limit of detection (LLD) for ATP was estimated for each microplate reader using the . Selection of Water Constant in PathCheck settings. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Or, with the reader specifications noted from the Instrument Description section of your reader's. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. ELISA Plate Readers / Microplate Readers. Prerequisites: SoftMax Pro 7. Table 1. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Next generation SpectraMax® absorbance microplate readers. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Microplate reader settings. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. A: Microplate Preparation. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. In addition, the SpectraMax® iD5 can be expanded to include bottom. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. 7°C (gradient heating design). Most papers they cultured bacteria to OD600>0. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Access this by going to Help >. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. When the user applies a set temperature of 37°C, the top is heated to 37. Molecular Devices SpectraMax M2E Microplate Reader. HTRF-compatible SpectraMax Multi-Mode Readers. Other technologies such as imaging. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Molecular Devices: Molecular Devices. 0 out of 5 $ 11,999. The dual monochromator optics allow. Step 2: Launch SoftMax Pro Software. Fluorescence Microplate Reader Comparison Chart. Omnia® Compatible Microplate Reader Documentation Version No. The guide also covers the software features, the specifications, and the accessories of the systems. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. Detailed specifications of each model reader are reported in Section 7, “Specifications”. A preconfigured protocol is available in the software to. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Please refer to the Obsolete Microplate Reader Models for more information. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Download PDF. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Microplate Readers BioTek Epoch 2 Microplate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 5 12. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. This pathlength correction is accomplished only with the use of. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. . With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. Molecular Devices. 380 nm/Exc. View the Molecular Devices website. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The advanced optical and. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. Also see:. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. To resume collecting kinetic measurements within the same file, press the Append button. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. 72 MB. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Table of Contents. Selection of Water Constant in PathCheck settings. 306 plate air cal. This unlocks the transport slide. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. 58% for the detection module and 2. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. In . The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Add to Cart. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 20 μL of Component A was added to each well of the microplate. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 305 reference level saturation. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Microplate automation and detection instruments for walk away operation and high quality results. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. The system uses two scanning monochromators to determine the optimal excitation and emission settings. 313 reference gain check fail. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It also integrates with the. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Add to Cart. 40266 Email: drugdiscoverytech@lifetech. microplate reader. The plate. 305 reference level saturation. Endpoint, kinetic, spectrum. The following document is intended to demonstrate setup of this instrument. . These lab workhorses are used in drug discovery, research, bioassay. Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 1. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. Fill a clean plate with water. Molecular Interactions. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Each sample has a discrete sample beam and reference beam so that each well is measured directly. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Example: [Drawer]. 1. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. 003 fmol 0. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The system uses two scanning monochromators to determine the optimal excitation and emission settings. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Wide range of applications The VersaMax reader covers. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. The plate reader optics are tunable for absorbance. The CellTiter-Glo assay from Promega uses luciferase. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. MPN:SpectraMax Plus. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. 0 out of 5 $ 5,999. SpectraDrop High-Throughput Screening (HTS) Kit. Compact and tunable absorbance microplate readers DATA SHEET. The 2-channel configuration is available with 0, 2, or 4 injectors. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. Figure 6: Transcreener ADP2 FI assay principle. Find regional support. flexibility of monochromator-based systems. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. 0 out of 5 $ 2,999-20%. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SpectraMax i3x Multi-Mode Detection Platform. Brochure. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. For readers without a cuvette port, these options are not shown in the software. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Introduction. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. B: Selection of Excitation Wavelength. . 1) Select the correct plate and the wells that you want to measure. SpectraMax® Microplate Readers and SoftMax® Pro Software. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. A. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Italic and boldface type are used for emphasis. com. Figure 1. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Cuvette Microplate wells. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Dual-Glo luciferase assay system (Promega Cat. We would like to show you a description here but the site won’t allow us. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Multimode Microplate Readers. ATP standard curves read on SpectraMax microplate readers. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 58% for the detection module and 2. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Transcreener ® ADP. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Devices’ StakMax® Microplate Stacker in a matter of minutes and . (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Assays can be better optimized by scanning across. The dual monochromator optics allow. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Fully tested with validations, warranty. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Request a Quote!Introduction. Create custom fluorescence assays without having to purchase expensive filter sets. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. With optimized reagents, option to operate the. Results are shown in Table 1. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Total volume (except deep. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Examples: “Press carefully to engage,” “ Do not. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. 4 μL DNA standards. Path Check Pathlength Measurement every Gemini EM Reader. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. In addition, the SpectraMax iD5 can be expanded to. Molecular Devices SpectraMax M2 Multimode Plate Reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Temperature control. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Step 2: Launch SoftMax Pro Software. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. For readers without a cuvette port, simply check the box next to ‘PathCheck’. . The advanced optical and. 6% for the on-board optics (monochromators). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The screw remains inside the instrument. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The instrument uses a paired monochromator and xenon flash. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The calculated LOQ values were 0. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Cell Cytotoxicity assay procedure. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. 3) This warranty. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Products : General, Microplate Readers. 2 only: SpectraMax Mini Update. Figure 1. SpectraMax M5 reader settings are shown. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. western blot membrane detection in a multimode microplate reader platform. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. 303 unable to cal dark current. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Molecular Devices. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices Spectramax iD3 Microplate Reader. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 5 87. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. 304 signal level saturation. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Blank subtraction and standard curve fitting were done automatically by the software. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 0200-6263. Type: Clear: Add to Cart. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Reporter Assay. 315 can't find zero order. Five preferred detection modes. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 7°C and the bottom to 36. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. All stand-alone instrument functions can be controlled using the software. 5 12. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast.